Directed transport of singlet excitation power is a key course of in pure light-harvesting techniques and a desired function in assemblies of practical natural molecules for natural electronics and nanotechnology purposes. Nevertheless, progress on this path is hampered by the shortage of ideas and mannequin techniques. Right here we reveal an all-optical strategy to control singlet exciton transport pathways inside supramolecular nanostructures by way of singlet–triplet annihilation, i.e., to implement an efficient movement of singlet excitons alongside a predefined path. For this proof-of-concept, we domestically photo-generate a long-lived triplet exciton inhabitants and subsequently a singlet exciton inhabitants on single bundles of H-type supramolecular nanofibres utilizing two temporally and spatially separated laser pulses. The native triplet exciton inhabitants operates as a gate for the singlet exciton transport since singlet–triplet annihilation hinders singlet exciton movement throughout the triplet inhabitants. We visualize this manipulation of singlet exciton transport by way of the fluorescence sign from the singlet excitons, utilizing a detection-beam scanning strategy mixed with time-correlated single-photon counting. Our reversible, all-optical manipulation of singlet exciton transport can pave the way in which to realising new design rules for practical photonic nanodevices.